《植物生理学报》 2014, 50(11): 1717-1720

樟叶越桔的组织培养与快速繁殖

罗旭璐¹, 唐军荣², 李娜¹, 丁勇¹, 张德国¹, 马焕成², 赵平^1,*

西南林业大学¹西南山地森林资源保育与利用省部共建教育部重点实验室, ²国家林业局西南地区生物多样性保育重点实验室, 昆明650224

通信作者：赵平；E-mail: hypzhao@yahoo.com；Tel: 0871-63863022

摘　要：

以野生樟叶越桔幼嫩带芽茎段为外植体, 通过对初代启动培养、增殖及生根培养方案的筛选, 建立了樟叶越桔的组织培养和快速繁殖体系。结果表明, 最适启动培养基为WPM+6-BA 3.0 mg•L^-1+NAA 0.2 mg•L^-1, 外植体诱导萌发率达83.33%; 增殖培养基为WPM+ZT 2.0 mg•L^-1, 可获得较高的增殖倍率, 且暗培养7 d可达最佳增殖效果; 生根最适培养基为WPM+IBA 0.2 mg•L^-1, 生根率达86.67%。

关键词：樟叶越桔; 茎段; 组织培养; 快速繁殖

收稿：2014-09-15   修定：2014-10-23

资助：国家自然科学基金(21462040和31460076)。

Tissue Culture and Rapid Propagation of Vaccinium dunalianum

LUO Xu-Lu¹, TANG Jun-Rong², LI Na¹, DING Yong¹, ZHANG De-Guo¹, MA Huan-Cheng², ZHAO Ping^1,*

¹Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, Ministry of Education, ²Key Laboratory of Biodiversity Conservation in Southwest China, State Forestry Administration, Southwest Forestry University, Kunming 650224, China

Corresponding author: ZHAO Ping; E-mail: hypzhao@yahoo.com; Tel: 0871-63863022

Abstract:

Taking the healthy tender stem segments of wild Vaccinium dunalianum as explants, the media for axillary bud induction, subculture and rooting culture were selected. The results showed that the optimal medium for initial culture with an induction rate of 83.33% was WPM+6-BA 3.0 mg•L^-1+NAA 0.2 mg•L^-1, and the optimal medium for subculture was WPM+ZT 2.0 mg•L^-1 with darkness cultured for 7 d. The optimal rooting medium was WPM+IBA 0.2 mg•L^-1, with a rooting rate of 86.67%.

Key words: Vaccinium dunalianum; stem segment; tissue culture; rapid propagation